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NZY Total RNA Isolation kit

Marka : NZYTech

Katalog No : MB13402

Description: NZY Total RNA Isolation kits are designed for the easy purification of total RNA of highest integrity (longer than 200 bases) from a variety of sources such as animal tissues, animal and human blood, bacteria cells and cell cultures. This method uses denaturing lysis buffer, containing guanidine thiocyanate, which inactivates cellular RNases to ensure preparation of intact RNA molecules. Contaminating DNA is removed by applying the sample into a homogenization column followed by addition of DNase I. Ethanol is added to provide selective binding of total RNA into the silica membrane column and impurities are efficiently washed away. High-quality RNA is then eluted in RNase-free water and it is ready to use for applications like Reverse Transcriptase (RT) PCR, qRT-PCR, in vitro translation or cDNA synthesis.
The NZY Total RNA Isolation kit is optimized to isolate up to 70 µg of RNA/column with an A260/280 ratio between 1.9 and 2.1 from up to 30 mg of animal tissue 1×109 bacteria cells or 1×106 of cell culture. We suggest not exceeding the maximum recommended starting material to prevent reduction in yield and purity of RNA isolated.

ADDITIONAL INFORMATION

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