Xho I
Marka : NZYTech
Katalog No : MB074
Description: Xho I is a Restriction Endonuclease purified from an Escherichia coli strain that carries the Xho I gene from Xanthomonas holcicola. Xho I activity is impaired by CpG methylation (CG methylase cleaves CT5mCGAG very slowly). NZYTech providesXho I with a specific buffer, NZYBuffer A. NZYTech’s Xho I is an enzyme that acts at 37 °C. For double digestions please use NZYBuffer U (not provided, MB1100).
Concentration: 10000 units/ml.
Reagents supplied with the enzyme: 10× NZYBuffer A.
Storage buffer: 25 mM NaHepes, pH 7.5, 500 mM NaCl, 150 mM imidazol, 2.5 mM CaCl2, 50% (v/v) glycerol.