+90 (312) 473 22 92 · · · · ·

NZY RNase H (E. coli)

Marka : NZYTech

Katalog No : MB085

Description: NZY RNase H is a recombinant endoribonuclease purified from anEscherichia coli strain that carries the cloned RNase H gene (rnh). The enzyme specifically hydrolyses the phosphodiester bonds of RNA which is hybridized to DNA. RNase H does not degrade single and double-stranded DNA or unhybridized RNA.  It is a key enzyme in the removal of mRNA after first-strand cDNA synthesis. In addition, RNase H is useful for the removal of poly(A) tails on mRNAs after hybridizarion with oligo (dT), and for oligodeoxyribonucleotide-directed site-specific cleavage of RNA.

Molecular Weight: 18.9 kDa

Shipping and Storage conditions: Shipped refrigerated. NZY RNase H should be stored at -20 °C in a freezer without a defrost cycle. The enzyme will remain stable up to 3 years if stored as specified.

Storage buffer: 20 mM Tris-HCl, pH 7.9, 100 mM KCl, 10 mM MgCl2, 0.1 mM EDTA, 0.1 mM DTT and 50% (v/v) glycerol.

Concentration: 5 U/µl

Reaction buffer (10×): 200 mM Tris-HCl, pH 8.4, 500 mM KCl, 50 mM MgCl2, 200 mM DTT.



MSDS (163,25 KB)

Product Brochure (114,39 KB)

Micro Counter 1000
Yeni Nesil Hücre Sayım Cihazı Simple Counter ile işiniz Artık Daha Kolay
Real-Time PCR
Applied Biosystems® VIIA ™7 Real-Time PCR Sistemi - 96 Well
Flow Sitometre
Flow Sitometre ve Aksesuarları