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NucView 488 and RedDot 2 Apoptosis and Necrosis Kit

Marka : Biotium

Katalog No : 30072

Product Description

NucView™488 and RedDot™2 Apoptosis/Necrosis Assay Kit contains NucView™488 Caspase-3 Substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot 2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry.

In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, NucView™488 Caspase-3 Substrate can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. The substrate consists of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with bright green fluorescence. Thus, NucView™488 Caspase-3 Substrate allows detection caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis.

RedDot™2 is a cell membrane-impermeable, far-red dye with high selectivity for membrane compromised or dead cells. The far-red emission of RedDot™2 is well-separated from the green fluorescence of NucView™488. RedDot™2 can be excited by wavelengths from 488 to 647 nm, and therefore can be used with the 488 nm flow cytometry laser line.

Note: while NucView™488 staining is formaldehyde-fixable and compatible with subsequent immunostaining, fixation after staining with RedDot™2 is not recommended because it can result in increased background staining of healthy cells.

 

Flyer (PDF): Apoptosis, Necrosis, and Cell Viability Assays web

 

MSDS (PDF): MSDS 30072

 

Protocol (PDF): PI-30072

(PDF): Validated cell lines for NucView 488 with references

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