CF™405M Annexin V conjugate is Annexin V labeled with our superior blue fluorescent dye CF™405M. Annexin V is a 35-36 kDa protein that has a high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the cytoplasmic surface of the cell membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, making itself available for Annexin V binding. CF™405M is a blue fluorescent dye spectrally similar to Pacific Blue dye. CF™405M is as bright as Pacific Blue dye but is significantly more photostable than the latter. An additional advantage of CF™405M is that it has less spillover in the 525/50 green channel than Pacific Blue dye due to narrower emission peak, making CF™405M a superior choice in multi-color detection applications. We also offer CF™405S, another 405 nm-excitable dye with an even shorter emission wavelength (see the CF™405S Flyer for details).
Flyer (PDF): FL-CF405M
MSDS (PDF): MSDS-CFAnnexinV
Protocol (PDF): PI-Annexin V