Brand :
Description: The Low Molecular Weight Protein Marker for SDS electrophoresis is a liquid mixture of seven purified proteins for use in molecular weight estimation in the presence of the detergent sodium dodecyl sulphate (SDS). This protein marker contains no oligosaccharides that may cause anomalous migrations and heterogeneous fuzzy bands. The molecular mass of the protein under investigation is determined by comparing its electrophoretic mobility with that of proteins contained in the kit. Three (MB08201) or six (MB08202) 500 µl vials are supplied, each containing a mixture of highly purified protein standards of molecular mass ranging from 18500 to 96000 when used in denaturing polyacrylamide electrophoresis. The mixture is supplied in a loading buffer for direct loading on gels.
Storage: The Low Molecular Weight Protein Marker should be stored at -20 °C. Preferably make 50-100 µl aliquots to avoid contamination of stock solution.
Components: Protein mixture (800 µg/vial) containing the following proteins (enough for 100 lanes):
Xylanase U4, Clostridium thermocellum, 60 µg, molecular weight (Mr) 96 000
Albumin, bovine serum, 180 µg, Mr 66 000
Phosphomanose Isomerase, Cellvibrio mixtus, 100 µg, Mr 48 000
Cellulase 5A, Cellvibrio mixtus, 110 µg, Mr 40 000
Cellulase M9, Clostridium thermocellum, 100 µg, Mr 32 000
Family 4 Carbohydrate esterase, Clostridium thermocellum, 220 µg, Mr 26 000
Xyloglucan-binding domain M6, Clostridium thermocellum, 30 µg, Mr 18 500
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