PMA dye 20 mM in H2O

Marka :

Katalog No : 40019

Product Description

PMA (propidium monoazide) dye is a DNA modifier invented by scientists at Biotium. It is a photo-reactive dye that binds to dsDNA with high affinity. Upon photolysis with visible light, PMA dye covalently attaches itself to dsDNA. The PMA-modified dsDNA cannot be amplified by PCR. The dye is designed to be cell membrane-impermeable. Thus, in a population of live and dead cells, only dead cells are susceptible to DNA modification due to compromised cell membranes. This unique feature of PMA dye makes it highly useful in selective detection of live bacteria by qPCR. A bacterial sample can be first pre-treated with PMA under light to inactivate dsDNA from dead cells. DNA is then extracted and analyzed by PCR. Since Biotium first developed PMA dye, there have been numerous publications on the use of the dye in pathogenic bacterial detection related to food and water safety, medical diagnosis and biodefense; download the PMA Reference List. Please also see PMA in lyophilized format, catalog no. 40013.

For photoactivation of PMA or EMA dye, we recommend the use of Biotium’s PMA-Lite LED Photolysis Device (E90002), which is designed to conduct photolysis under controlled conditions.

Principle of selective detection of live cells using PMA dye.

• Molecular weight: 511
• Abs = 464 nm (before photolysis)
• Abs/Em = 510/610 nm (following photolysis and covalent attachment to DNA/RNA)
• Dark red solid soluble in DMSO or DMF
• Store at 4°C and protect from light at all times

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  Flyer (PDF): FL-PMA and PMA kits

   

  MSDS (PDF): MSDS 40019

   

  Protocol (PDF): PI-40013, 40019

  Reference List (PDF): PMA Reference List