Klenow Fragment of DNA Polymerase I

Brand :

Catalog No : MB00901

Description: A truncated fragment of DNA polymerase I that lacks the 5' to 3' exonuclease activity of the full length enzyme. The enzyme is well suited for use in 3'-end labelling of DNA fragments for sequence analysis, for the conversion of 5'-extended ends to flush ends for cloning, for second-strand synthesis of cDNA and for random primer labelling of DNA fragments.

Storage conditions: 50 mM potassium phosphate, pH 7.0, 1.0 mM DTT, 50% (v/v) glycerol. Store at -20 °C.

Concentration: 5 U/µl.

10x reaction buffer: 500 mM Tris-HCl, pH 7.5, 100 mM MgCl₂, 10 mM DTT, 500 µg/ml BSA.