Speedy Ligase

Brand :

Catalog No : MB130

Description: Speedy ligase is a T4 DNA ligase developed to carry out fast (less than 15 minutes) and efficient ligation of sticky-end and blunt-end DNA at room temperature. The enzyme catalyses the formation of a phosphodiester bond between juxtaposed 5'-phosphoryl and 3'-hydroxyl termini in duplex DNA. Rapid ligation is based on the combination of Speedy ligase with a 4× Speedy ligation buffer.

Storage conditions: 20 mM Tris-HCl, pH 7.6, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA and 50% (v/v) glycerol. Store at -20 °C in a non-frost free freezer. 

Inactivation: Speedy ligase is heat inactivated at 65 °C for 10 min.

Concentration: 10 U/µ