T4 Polynucleotide Kinase

Brand : NZYTech

Catalog No : MB00801

Description: T4 PNK catalyzes the transfer of the terminal phosphate of ATP to 5'-hydroxyl termini of polynucleotides such as DNA and RNA, oligonucleotides and 3'-mononucleotides. In the presence of ADP, it will also catalyze the exchange of 5'-terminal phosphate groups and ATP. This enzyme also possesses a 3'-phosphatase and 2', 3' cyclic phosphodiesterase activity.

Storage conditions: 50 mM Tris-HCl, pH 7.6, 1.0 mM DTT, 0.1 mM EDTA and 50% (v/v) glycerol. Store at -20 °C.

Concentration: 30 U/µl.

10x reaction buffer: 0.5 M Tris-HCl, pH 7.6, 100 mM MgCl₂, 100 mM 2-mercaptoethanol.

Supplied with dilution buffer: 50 mM Tris-HCl, pH 8.0.