Description:

NZY Plant/Fungi gDNA Isolation kits are designed for the simple and rapid small-scale preparation of highly pure genomic DNA from plant tissues and fungi samples. The method is spin column silica-based and requires no phenol or chloroform extraction. This kit uses two optimized lysis buffers systems based on the established CTAB and SDS methods. NZY Homogenization columns are included in this kit for conveniently clearing the crude lysates. The clear flow-through is mixed with Binding buffer PN for optimal binding of DNA to the silica membrane. Then, the DNA is selectively absorbed into the NZYSpin Plant Column and others impurities such as proteins and salts are removed during the washing steps. The eluted genomic DNA has a A260/280 ratio between 1.7 and 1.9 what makes it ready to use in applications like sequencing, PCR, multiplex-PCR, genotyping and a wide range of other enzymatic manipulations.

The NZY Plant gDNA Isolation kit is optimized to isolate up to 30 µg of DNA from up to 100 mg (wet weight) or 20 mg (dry weight) of plant/fungi samples, depending of the plant/fungi species. We suggest not using more than the recommended starting material to prevent reductions in yield and purity.

 

ADDITIONAL INFORMATION

MSDS (0,53 MB)Product Brochure (357,90 KB)