NZY Tissue gDNA Isolation kit

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Catalog No : MB135

Description: NZY Tissue gDNA Isolation kits are designed for the simple and rapid small-scale preparation of highly pure genomic DNA from a variety of sample sources including animal tissues and cells, Gram-positive and Gram-negative bacteria, mouse tails, yeast, forensic samples and clinical samples. The method is spin column silica-based and requires no phenol or chloroform extraction. This kit uses optimized lysis buffers containing Proteinase K and SDS to release DNA from cells. After preparing the lysate, DNA is selectively absorbed in the NZYSpin Tissue Column and others impurities such as proteins and salts are removed during the washing steps. The eluted genomic DNA has a A_260/280ratio between 1.7 and 1.9 what makes it ready to use in applications like sequencing, PCR, multiplex-PCR, genotyping and a wide range of other enzymatic manipulations.
The NZY Tissue gDNA Isolation kit is optimized to isolate up to 35 µg of DNA from up to 25 mg of tissue samples or 10⁷ cells. We suggest not using more than the recommended start material to prevent reduction in yield and purity of DNA isolated. For samples with very high RNA and protein contents (e.g. liver or spleen tissues), use only up to 15 mg of the sample. This kit is suitable for isolation of DNA from human or animal blood.

ADDITIONAL INFORMATION

Product Brochure (350,97 KB)Support Protocol - insects (166,75 KB)Support Protocol - paraffin tissues (171,63 KB)Support Protocol - stool (173,46 KB)Support Protocol - swabs (178,13 KB)Support Protocol - yeast (176,59 KB)MSDS (0,54 MB)