Description: NZYGelpure kit is designed for the purification of DNA from TAE/TBE agarose gels and for the direct purification of PCR products. The kit can be used to purify DNA fragments from 100 bp to 10 kb. NZYGelpure purification kit utilizes a silica-gel based membrane which selectively adsorbs up to 20 µg of DNA fragments in the presence of specialized binding buffers. Soluble agarose, nucleotides, oligos (<30mer), enzymes, mineral oil and other impurities do not bind to the membrane and are washed away. DNA fragments are then eluted off the column and can be used for downstream protocols without further processing. Binding Buffer contains a pH indicator, allowing evaluation of optimal pH for DNA binding. The pH indicator does not interfere with DNA binding.
Storage: All kit components can be stored at room temperature (20-25 °C) and are stable for up to one year. For longer storage, keep all contents at 4 °C. Add 32 ml (MB01101) or 100 ml (MB01102) of ethanol to each bottle of Wash buffer.