Description: Nt.BbvCI is a Nicking Endonuclease purified from an Escherichia colistrain that carries the BbvCI fragment from Brevibacillus brevis (Bacillus brevis). The enzyme cleaves only one strand of DNA on a double-stranded DNA substrate. Nt.BbvCI activity is blocked by some combinations of overlapping CpG methylation. NZYTech provides Nt.BbvCI with a specific reaction buffer. NZYTech’s Nt.BbvCI Nicking Endonuclease is an enzyme that acts at 37 °C.

 

Concentration: 10000 U/mL.

 

Reagents supplied with the enzyme: 10× Nt.BbvCI Buffer.

 

Storage buffer: 10 mM Tris-HCl, pH 7.4, 50 mM KCl, 1 mM Dithiothreitol, 0.1 mM EDTA, 0.2 mg/mL BSA, 50% (v/v) glycerol.