Description: NZYTech’s Speedy restriction enzymes are a new generation of DNA modifying enzymes developed for rapid DNA digestion. All Speedy enzymes are 100% active in the NZYSpeedyBuffers and are able to digest DNA in 5-15 minutes. NZYSpeedyBuffers are universal and enable any combination of NZY Speedy restriction enzymes to work simultaneously. It’s ideal for double or multiple digestions, eliminating the need for sequential digestions. NZYSpeedyBuffer Orange also allows ready gel load after digestion. Speedy Bgl II activity is not blocked by overlapping dam methylation (G^mATC) as well as by dcm and CpG methylations. NZYTech’s Speedy Bgl II is a fast enzyme that performs its reaction usually in 5 minutes at 37 °C.

 

Concentration: One μL of enzyme can completely digest up to 1 μg of DNA in 5 min.

 

Reagents supplied with the enzyme: 10× NZYSpeedyBuffer Colourless

     10× NZYSpeedyBuffer Orange

 

Storage buffer: 25 mM NaHepes, pH 7.5, 500 mM NaCl, 150 mM imidazol, 2.5 mM CaCl₂, 50% (v/v) glycerol.