Description: NZYTech’s Speedy restriction enzymes are a new generation of DNA modifying enzymes developed for rapid DNA digestion. All Speedy enzymes are 100% active in the NZYSpeedyBuffers and are able to digest DNA in 5-15 minutes. NZYSpeedyBuffers are universal and enable any combination of NZY Speedy restriction enzymes to work simultaneously. It’s ideal for double or multiple digestions, eliminating the need for sequential digestions. NZYSpeedyBuffer Orange also allows ready gel load after digestion. Speedy Nco I activity is not affected by dam methylation, dcm methylation and CpG methylation. NZYTech’s Speedy Nco I is a fast enzyme that performs its reaction usually in 15 minutes at 37 °C.

Concentration: One μL of enzyme can completely digest up to 1 μg of DNA in 15 min.

Reagents supplied with the enzyme: 10× NZYSpeedyBuffer Colourless
                                                                     10× NZYSpeedyBuffer Orange

Storage buffer: 25 mM NaHepes, pH 7.5, 500 mM NaCl, 150 mM imidazol, 2.5 mM CaCl₂, 50% (v/v) glycerol.

 

ADDITIONAL INFORMATION

Product Brochure (361,58 KB)MSDS enzyme (120,95 KB)MSDS buffer 1 (101,32 KB)MSDS buffer 2 (101,46 KB)