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Speedy Sfi I

Brand : NZYTech

Catalog No : MB15001

Description: NZYTech’s Speedy restriction enzymes are a new generation of DNA modifying enzymes developed for rapid DNA digestion. All Speedy enzymes are 100% active in the NZYSpeedyBuffers and are able to digest DNA in 5-15 minutes. NZYSpeedyBuffers are universal and enable any combination of NZY Speedy restriction enzymes to work simultaneously. It’s ideal for double or multiple digestions, eliminating the need for sequential digestions. NZYSpeedyBuffer Orange also allows ready gel load after digestion. Speedy Sfi I activity is often affected by CG methylase. When the sequence containing the recognition site is as follows, the enzyme can be affected by dcm methylase and would not digest DNA substrates:

GGCCWGGNNGGCC
GGCCNNNNNGGCCWGG
 
NZYTech’s Speedy Sfi I is a fast enzyme that performs its reaction usually in 5 minutes at 50 °C.
 
Concentration: One μL of enzyme can completely digest up to 1 μg of DNA in 5 min.
 
Reagents supplied with the enzyme: 10× NZYSpeedyBuffer Colourless
     10× NZYSpeedyBuffer Orange
 
Storage buffer: 10 mM Tris-HCl, pH 7.5, 200 mM NaCl, 0.1 mM EDTA, 7 mM 2-mercaptoethanol, 200 µg/mL (w/v) BSA, 50% (v/v) glycerol.
 

ADDITIONAL INFORMATION

Product Brochure (361,29 KB)MSDS enzyme (488,32 KB)MSDS buffer 1 (101,32 KB)MSDS buffer 2 (101,46 KB)

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