|
Description: NZY Ribonuclease Inhibitor is a recombinant inhibitor of ribonucleases (RNases; EC 3.1) of the pancreatic type, such as RNase A, RNase B and RNase C, purified from Escherichia coli. This protein is useful in any application where eukaryotic RNase contamination is a potential problem. This inhibitor can be used to protect template RNA in cDNA synthesis reactions or in vitro transcription/translation. It is not effective against RNase 1, RNase T1, RNase T2, S1 nuclease, RNase H or RNase fromAspergillus sp.
Shipping and storage conditions: Shipped on dry ice. The enzyme should be stored at -20 °C in a freezer without defrost cycles. NZY Ribonuclease Inhibitor will remain stable up to 3 years if stored as specified.
Storage buffer: 20 mM Tris-HCl, pH 8.0, 50 mM KCl, 0.5 mM EDTA, 8 mM DTT and 50% (v/v) glycerol.
Concentration: 40 U/μl.
|