Description:
The NZY First-Strand cDNA Synthesis Kit, separate oligos, is a system that includes all the necessary components to synthesize first-strand cDNA, except template RNA. Random hexamers and Oligo(dT)18 primers are provided in separate tubes to offer the convenience to choose the appropriate primer to initiate your reverse-transcription reaction.
The resulting single-stranded cDNA is suitable for use in real-time quantitative Reverse Transcription PCR (RT-qPCR). NZY First-Strand cDNA Synthesis Kit, separate oligos, is formulated to provide high yields of full-length cDNA products and to increase sensitivity in RT-qPCR.
Starting material can range from 1 ng up to 5 µg of total RNA. Besides random hexamers and Oligo(dT)18 primers, the kit includes NZYRT 2× Master Mix, no oligos, which contains dNTPs, MgCl2 and an optimized RT buffer; 10× Annealing Buffer and NZYRT Enzyme Mix. The NZYRT Enzyme Mix includes both the NZY Reverse Transcriptase (RNase H minus) and the NZY Ribonuclease Inhibitor in order to protect RNA against degradation due to ribonuclease contamination. RNase H (from E. coli) is provided in a separate tube to specifically degrade the RNA template in cDNA:RNA hybrids after the first-strand cDNA synthesis. This procedure will improve the sensitivity of subsequent RT-qPCR reactions since PCR primers will bind more easily to the cDNA.
Shipping conditions:
NZY First-Strand cDNA Synthesis Kit, separate oligos, is shipped on dry ice.
Storage conditions:
Store all kit components at -20 °C in a freezer without defrost cycles. Stability can be extended by storing it at -80 °C.
Brand : 
+90 (312) 473 22 92 · 
