Description:

The NZY M-MuLV First-Strand cDNA Synthesis Kit is a system that includes all the necessary components to synthesize first-strand cDNA, except template RNA. 

The resulting single-stranded cDNA is suitable for use in real-time quantitative Reverse Transcription PCR (RT-qPCR). NZY M-MuLV First-Strand cDNA Synthesis Kit is formulated to provide high yields of full-length cDNA products and to increase sensitivity in RT-qPCR.

Starting material can range from 1 ng up to 5 µg of total RNA. The kit includes a combination of random hexamers and oligo(dT)18 primers in order to increase sensitivity. The primers are included in the NZYRT 2× Master Mix, which also contains dNTPs, MgCl₂ and an optimized RT buffer. NZYM-MuLV RT Enzyme Mix includes both NZY M-MuLV Reverse Transcriptase (RNase H minus) and NZY Ribonuclease Inhibitor in order to protect RNA against degradation due to ribonuclease contamination. RNase H (from E. coli) is provided in a separate tube to specifically degrade the RNA template in cDNA:RNA hybrids after the first-strand cDNA synthesis. This procedure will improve the sensitivity of subsequent RT-qPCR reaction since PCR primers will bind more easily to the cDNA.

 

Shipping conditions:

NZY M-MuLV First-Strand cDNA Synthesis Kit is shipped on dry ice.

 

Storage conditions:

Store all kit components at -20 °C in a freezer without defrost cycles. Stability can be extended by storing it at -80 °C.

ADDITIONAL INFORMATION

Product Brochure (388,21 KB)MSDS (0,54 MB)