GelGreen™ is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye designed to stain either dsDNA, ssDNA or RNA in agarose gels. GelGreen is far more sensitive than SYBR Safe (Figure 1). Unlike SYBR dyes, which are known to be unstable, GelGreen is very stable, both hydrolytically and thermally. GelGreen has a UV absorption between 250 nm and 300 nm and a strong absorption peak centered around 500 nm (Figure 2). Thus, GelGreen is compatible with either a 254 nm UV transilluminator or a gel reader equipped with visible light excitation (such as a 488 nm laser-based gel scanner or a Dark Reader).
GelGreen can be used for either post gel staining or precast gel staining.
A series of safety tests have confirmed that GelGreen™ is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, GelGreen can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal. For detailed test results, you may download a complete GelRed/GelGreen Safety Report here.
GelGreen™ 10,000X solution in water is our latest formulation that eliminates the hazards of handling DMSO for better safety, and is recommended for new users of GelGreen™. We continue to offer GelGreen™ 10,000X solution in DMSO for established users who do not wish to change their existing laboratory protocols.
FEATURES
Shown by the Ames test and other tests to be nonmutagenic and noncytotoxic
Passed environmental safety tests for direct disposal down the drain or in regular trash
Much more sensitive than EtBr and SYBR Safe
Available in water, stable at room temperature for long-term storage and microwavable |
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Very simple procedures for either precast or post gel staining
GelGreen replaces SYBR or GelStar with no optical setting change (see Figure 2)
Compatible with downstream DNA manipulations such as restriction digest, sequencing and cloning. Flyer (PDF): GelRed & GelGreen Flyer MSDS (PDF): MSDS 41005 Protocol (PDF): PI-41005 Safety Report (PDF): GR & GG safety |
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