GelRed Nucleic Acid Gel Stain, 10,000X in DMSO

Brand :

Catalog No : 41002, 41002-1

Product Description

GelRed™ is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed™ is far more sensitive than EB without requiring a destaining step (Figure 1). GelRed™ and EB have virtually the same spectra (Figure 3), so you can directly replace EB with GelRed™ without changing your existing imaging system.

GelRed™ can be used to stain dsDNA, ssDNA or RNA in agarose gels via either precast or post gel staining. GelRed can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gels via post gel staining. GelRed is also compatible with downstream DNA manipulations such as restriction digest, sequencing and cloning.

A series of safety tests have confirmed that GelRed™ is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, GelRed™ can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal. For detailed test results, you may download the GelRed/GelGreen Safety Report.

For new users, Biotium recommends GelRed™ 10,000X solution in water (41003), our latest formulation that eliminates the hazards of handling DMSO for better safety. We continue to offer GelRed™ 10,000X solution in DMSO for established users of GelRed™ in DMSO who do not wish to change their laboratory protocols. The performance and stability of GelRed™ 10,000X is comparable in both the water and DMSO formulations. For your convenience, we also offer ready-to-use GelRed™ 3X in water (41001) that can be directly used for post gel staining. For customers who look for large pack size, we offer a cost-saving bulk pack size of 10 mL.

FEATURES

• Safer than EB: Shown by the Ames test and other tests to be nonmutagenic and noncytotoxic
• Easy disposal: Passed environmental safety tests for direct disposal down the drain or in regular trash
• Ultra-sensitive: Much more sensitive than EtBr and SYBR Safe
• Extremely stable: Available in water, stable at room temperature for long-term storage and microwavable
• Simple to use: Very simple procedures for either precast and post gel staining
• Perfectly compatible with a standard UV transilluminator : GelRed replaces EtBr with no optical setting change; GelGreen replaces SYBR or GelStar with no optical setting change (see Figure 3)
• Perfectly compatible with downstream applications : Compatible with downstream DNA manipulations such as restriction digest, sequencing and cloning.

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  Flyer (PDF): GelRed & GelGreen Flyer

   

  MSDS (PDF): MSDS 41002

   

  Protocol (PDF): PI-41002

   

  Safety Report (PDF): GR & GG safety