Lumitein Protein Gel Stain 1X

Brand :

Catalog No : 21001, 21001-1, 21001-2

Product Description

Lumitein™ is a luminescent dye designed for detecting proteins in SDS polyacrylamide (SDS-PAGE) gels, but that can also be used to detect proteins in native PAGE gels after an additional SDS incubation step. Lumitein™ is the only protein gel stain that combines superior sensitivity, staining speed, ease of use and compatibility. It is as sensitive as the best silver stain by detecting 1 ng or less protein (Figure 1). Unlike silver stain, however, Lumitein™ has a linear detection range of at least 3 orders of magnitude. It is among the simplest protein gel stain by staining protein in gels in 90 minutes or less time without a separate fixation step. Lumitein™ has an excitation spectrum that makes detection possible with either a simple UV box or a high-end laser scanner. Moreover, protein gel staining with Lumitein™ is compatible with downstream protein analyses such as mass spectrometry and Edman-based sequencing (Figure 2).

Lumitein™ protein gel stain is available as either a 100X concentrated solution or 1X solution. The 100X solution (cat#21002), which can be readily diluted to the 1X working solution with methanol, acetic acid, and water, is designed to save shipping cost while the ready-to-use 1X solution is for ultimate user convenience.

 Highly Sensitive: At least as sensitive as silver stain by detecting < 1 ng protein.

 Extremely Simple & Fast: Fixation and staining in a single 30- or 90-min combined step followed by a simple rinse in water.

 Very Low Background

 Excellent Instrument Compatibility: Can be used with either a simple 300 nm UV Box (EtBr filter), a Dark Reader, or a high-end laser scanner.

 Wide Linear Detection Range : At least three orders of magnitude.

 Compatible with Downstream Analysis: Compatible with MS and sequencing.