Brand : This kit contains the NucView™488 caspase-3 substrate and the MitoView™633 mitochondrial dye. The kit provides a convenient tool for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using either fluorescence microscopy or flow cytometry. The bi-functional NucView™488 caspase-3 substrate is able to detect both intracellular caspase-3 and at the same time stain the cell nucleus, which is known to undergo morphological changes during apoptosis. MitoView™633 is a new far-red fluorescent mitochondrial dye, which diffuses across the plasma membrane and accumulates in the mitochondria. The staining is dependent upon the mitochondrial membrane potential; thus, apoptotic cells exhibit a much lower MitoView™633 dye staining compared to healthy cells. Furthermore, the spectral separation of these two dyes minimizes fluorescence overlap.
Untreated Jurkat cells stained with the NucView 488 and MitoView 633 Apoptosis Kit. Healthy cells with intact mitochondrial membrane potential stain with far red MitoView 633 mitochondrial membrane potential dye (red), while apoptotic cells stain green with NucView 488 caspase-3 substrate, and do not stain with MitoView 633.
Flyer (PDF): Apoptosis, Necrosis, and Cell Viability Assays web
Protocol (PDF): 30062
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