Brand : NucView™ 488 Caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3/7 activity within live cells in real time.
Unlike conventional caspase assays, NucView™ 488 Caspase-3 substrate detects caspase-3/7 activity within individual intact cells without inhibiting caspase activity. The substrate consists of a fluorogenic DNA dye and a DEVD substrate moiety specific for caspase-3/7. The substrate, which is both non-fluorescent and nonfunctional as a DNA dye, rapidly crosses cell membranes to enter the cytoplasm, where it is cleaved by caspase-3 to form a high-affinity DNA dye that stains the nucleus bright green. Thus, the NucView™ 488 caspase-3 substrate is bi-functional, allowing detection of intracellular caspase-3/7 activity and visualization of changes in nuclear morphology during apoptosis. The fluorescent staining produced in response to caspase-3/7 activity is compatible with subsequent fixation and permeabilization for immunostaining.
In addition to stand-alone substrate stock solutions, we offer NucView™ 488 assay kits with detailed application protocols.
NucView™ enzyme substrate technology is covered by U.S. Patent No. 8,092,784.
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NucView™488 & CF™ 647 Annexin V
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