Description:

NZY Auto-Induction Kit is an innovative product to supplement your specific culture medium that was developed to grow E. coli to high cell densities while promoting auto-induction of recombinant gene expression. The NZY Auto-Induction Kit contains two concentrated sterile solutions and was designed for IPTG-inducible bacterial expression systems. Addition of these solutions to traditional complex media, such as Luria-Bertani (LB) broth, Terrific Broth (TB), results in high-level protein production. The kit simplifies induction of expression constructs by eliminating the need to take repeated OD readings and IPTG addiction. The method is based on medium components that are metabolized differentially to promote culture growth to high cell densities and subsequent induction of protein expression from lac-based promoters. NZY Auto-Induction Kit is ideal for high-throughput (HTP) methods, when you have to grow multiple cultures expressing various proteins simultaneously. 

NZYAI Solution 1 is the induction solution. It also contains magnesium and a mixture of trace metals. NZYAI Solution 2 is an optimized buffering solution.

 

Shipping conditions:

NZY Auto-Induction Kit is shipped at room temperature.

 

Storage conditions:

Store the NZY Auto-Induction Kit dry at room temperature. Once opened, store NZYAI Solution 1 at 4 °C. The solutions are provided sterile, so it is important to ensure sterile conditions of storage and handling.