BL21(DE3)
Brand : NZYTech
Catalog No : MB006
Description: BL21(DE3) Competent Cells are chemically competent Escherichia colicells used for protein expression in T7 RNA Polymerase-based systems. These cells are resistant to the lytic bacteriophages T1 and T5. The BL21 (DE3) strain is a derivative ofE. coli B. It is deficient in both lon and ompT proteases resulting in superior isolation of intact recombinant proteins. The host is a lysogen of DE3 and, therefore, carries a chromosomal copy of the T7 RNA polymerase gene that is controlled by the lacUV5 promoter. The strain utilizes the T7 RNA promoter to control protein expression. IPTG is used to induce expression of the T7 RNA polymerase.
Genotype: F¯ ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
Shipping and Storage: The BL21(DE3) Chemically Competent Escherichia coli cells are shipped on dry ice. Upon receipt, store at -80 °C.
System Components: BL21(DE3) Competent Cells (10 or 20 x 200 µL) Competent Cells Control Plasmid (10 µL at 0.1 ng/µL)
Quality Control: BL21(DE3) Competent Cells consistently yield > 1.0 × 106 colony-forming units/µg competent cells control plasmid when transformed with non-saturating amounts of DNA (0.2 ng/100 µL cells).
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