NZYSpeedy DNA polymerase

Marka :

Katalog No : MB108

Description: NZYSpeedy DNA polymerase is a recombinant thermostable DNA polymerase displaying a faster polymerization reaction than any other conventional non-proofreading enzyme. NZYSpeedy DNA polymerase is suitable to amplify target DNA sequences up to 3 kb in size and it is useful for fast routine DNA amplifications or genotyping. Only 5 seconds are required for the successful synthesis of 1 kb size DNA. NZYSpeedy DNA polymerase lacks 3´-5´ exonuclease activity. Resulting polymerase chain reaction (PCR) products have an A overhang and are suitable for cloning with NZYTech´s NZYPCR cloning kit (MB01301 or MB01302).

Storage temperature: NZYSpeedy DNA polymerase should be stored at -20 °C, in a constant temperature freezer. NZYSpeedy DNA polymerase will remain stable up to 3 years if stored as specified.

Storage buffer: 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% (v/v) glycerol.

Enzyme concentration: 5 U/μl

Reaction buffer (10x): 670 mM Tris-HCl, pH 8.8, 160 mM (NH₄)₂SO₄, 0.1% (v/v) Tween 20. Vortex the reaction buffer solution thoroughly after thawing and prior to use. Repeated freeze-thaw cycles will affect the stability of the buffer (the buffer will remain stable at +4 °C up to one month).

Magnesium Chloride solution: 50 mM MgCl₂. Provided to allow users to optimize MgCl₂ concentrations (1.5-4.0 mM). Vortex the MgCl₂ solution thoroughly after thawing and prior to use.