NZYSpeedy Proof DNA polymerase

Marka :

Katalog No : MB109

Description: NZYSpeedy Proof DNA polymerase is a recombinant thermostable DNA polymerase purified from Escherichia coli that combines high fidelity and fast polymerization reaction. NZYSpeedy Proof DNA polymerase possesses 3´-5´ exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. The error rate of the enzyme is similar to that of Pfu and KodDNA polymerases and significantly lower than the error rate of Taq DNA polymerases. NZYSpeedy Proof DNA polymerase is highly efficient in the amplification of DNA fragments up to 3 Kb in a short reaction period. Only 15 secondsare required for the successful synthesis ok 1 kb size DNA. NZYSpeedy Proof DNA polymerase generates blunt-ended polymerase chain reaction (PCR) products that are suitable for cloning with NZYTech´s NZYPCR cloning kit (MB01301 or MB01302).

Storage temperature: NZYSpeedy Proof DNA polymerase should be stored at -20 °C, in a constant temperature freezer. NZYSpeedy Proof DNA polymerase will remain stable up to 3 years if stored as specified.

Storage buffer: 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% (v/v) glycerol.

Enzyme concentration: 2.5 U/μl

Reaction buffer (10x): 1.2 M Tris-HCl, pH 8.0, 60 mM (NH₄)₂SO₄, 100 mM KCl, 0.01% (w/v) nuclease-free BSA, 1% (v/v) Triton X-100. Vortex the Reaction Buffer solution thoroughly after thawing and prior to use. Repeated freeze-thaw cycles will affect the stability of the buffer (the buffer will remain stable at +4 °C up to one month).

Magnesium Chloride solution: 50 mM MgCl₂. Provided to allow users to optimize MgCl₂ concentrations (1.5-4.0 mM). Vortex the MgCl₂ solution thoroughly after thawing and prior to use.