NZYTaq with 5x Gel Load Reaction Buffer

Marka :

Katalog No : MB038

Description: NZYTaq DNA polymerase is a recombinant modified form of Taq DNA polymerase purified from Escherichia coli. NZYTaq is provided with 5× Gel Load Reaction Buffer allowing reactions to be loaded directly onto gels without loading dye. The blue dye migrates at the same rate as a 3-5 kb DNA fragment in a 1% (w/v) agarose gel. The yellow dye migrates at a rate faster than primers (<50 bp) in a 1% (v/v) agarose gel. The buffer does not contain magnesium, allowing easy optimization in amplification reactions. The 5× Gel Load Reaction Buffer is not suitable when direct fluorescent or absorbance readings are required without prior purification of the amplified DNA from PCR. NZYTaq DNA polymerase lacks 3´-5´ exonuclease activity. Resulting polymerase chain reaction (PCR) products have an A overhang and are suitable for cloning with NZYTech´s NZYPCR cloning kit (MB01301 or MB01302).

Storage temperature: NZYTaq DNA polymerase should be stored at -20 °C, in a constant temperature freezer. NZYTaq DNA polymerase will remain stable if stored as specified.

Storage buffer: 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% (v/v) glycerol.

Enzyme concentration: 5 U/μl

Gel Load Reaction Buffer (5×): Proprietary formulation supplied at pH 8.8. Vortex the reaction buffer solution thoroughly after thawing and prior to use. Repeated freeze-thaw cycles will affect the stability of the buffer (the buffer will remain stable at 4 °C for at least of one month).

Magnesium Chloride solution: 50 mM MgCl₂. Provided to allow users to optimize MgCl₂ concentrations (1.5-4.0 mM). Vortex the MgCl₂ solution thoroughly after thawing and prior to use.