Supreme NZYTaq DNA polymerase

Marka :

Katalog No : MB079

Supreme NZYTaq DNA polymerase contains a recombinant modified form of Taq DNA polymerase  with a hot-start like PCR capacity. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer-dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme is restored during a short 5-minute incubation step at 95 °C. This highly robustTaq polymerase is a broad range enzyme suitable for a variety of general PCR applications to amplify target DNA sequences up to 3 kb in size. It enhances many complex PCR reactions by increasing both specificity and yield in a range of DNA concentrations up 5-10 pg. The activated enzyme maintains the same functionality as TaqDNA polymerase. Supreme NZYTaq DNA polymerase lacks 3´-5´ exonuclease activity. Thus, resulting polymerase chain reaction (PCR) products have an A overhang and are suitable for cloning with NZYTech´s NZY-A PCR cloning kits (MB05301 or MB05302). Supreme NZYTaq DNA polymerase allows an easy handling and a room-temperature setup.

Storage temperature: Supreme NZYTaq DNA polymerase should be stored at -20 °C, in a constant temperature freezer. Supreme NZYTaq DNA polymerase will remain stable up to 3 years if stored as specified.

Storage buffer: 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% (v/v) glycerol.

Enzyme concentration: 5 U/μl

Reaction buffer (10×): 670 mM Tris-HCl, pH 8.8, 160 mM (NH₄)₂SO₄, 400 mM KCl, 0.1% Tween 20. Vortex the reaction buffer solution thoroughly after thawing and prior to use. Repeated freeze-thaw cycles will affect the stability of the buffer (the buffer will remain stable at +4 °C up to one month).

Magnesium Chloride solution: 50 mM MgCl₂. Provided to allow users to optimize MgCl₂ concentrations (1.5-4.0 mM). Vortex the MgCl₂ solution thoroughly after thawing and prior to use.