NZYTaq 5x Optimizer Solution

Marka :

Katalog No : MB060

Description: NZYTaq 5× Optimizer Solution was developed to improve the efficacy and reduce the levels of non-specific amplifications of particular difficult PCR reactions using NZYTaq DNA polymerase. The solution contains a proprietary range of additives and stabilizers that have proven particularly useful when attempting to amplify ancient DNA or templates contaminated with PCR inhibitors. It is also useful for amplification of nucleic acids with high GC% (50-70%). The solution should be diluted 5× in the PCR reactions 

Storage temperature: NZYTaq 5× Optimizer Solution should be stored at -20 °C, in a constant temperature freezer. 

Functional assay: NZYTaq 5× Optimizer Solution was tested for performance in particular difficult amplifications using Escherichia coli genomic DNA. PCR performance was analysed in GreenSafe (MB08801)-stained agarose gel.

Nuclease assays: NZYTaq 5× Optimizer Solution was incubated with 0.5 μg of pUC18 for 2 hours at 37 °C or 72 °C. No nicking activity was observed after agarose gel electrophoresis.