NZYMutagenesis kit

Marka :

Katalog No : MB012

Description: NZYMutagenesis kit is designed to make point mutations and delete or insert single or multiple nucleotides in a DNA sequence. The system requires the provision of two synthetic oligonucleotide primers containing the desired mutation. Incorporation of the oligonucleotide primers with NZYProof DNA polymerase generates a mutated plasmid containing staggered nicks, which resists Dpn I digestion (as the synthetic DNA is not methylated). The resulting mutated plasmid is recovered through transformation of NZYStar competent cells. DNA isolated from dam- Escherichia coli strains, including JM101 and SCS110, is not a suitable template for the mutagenesis reaction.

ADDITIONAL INFORMATION

MSDS MB01201 & MB1203 (252,19 KB)MSDS MB01202 & MB1204 (311,47 KB)Product Brochure (0,51 MB)