NZYMidiprep, no columns
Marka :
Katalog No : MB14101
Description: NZYMidiprep kit is designed for the rapid, medium-scale preparation of highly pure plasmid DNA (typically 100 µg) from recombinant Escherichia coli strains. Plasmid DNA binds selectively to NZYTech columns (not provided) charged with a silica-based anion-exchange resin. All contaminants, such as proteins, RNA, salts, nucleotides and oligos (<40-mer) are washed from the column (not provided). In the elution step, the positive charge of the resin is neutralized by a pH shift to slightly alkaline conditions and pure plasmid DNA is eluted in a high-salt elution buffer. The purified nucleic acid products are suitable for use in the most demanding molecular biology applications, including transfection, in vitro transcription, automated fluorescent or manual sequencing, cloning, PCR and hybridization. To isolate DNA from low copy number plasmids, BACs or cosmids, or to obtain higher DNA concentrations, use 100 mL of E. coli cultures and double the volumes of Buffers M1, M2 and M3. This kit does not include the purification columns.
Storage: All kit components can be stored at room temperature (20-25 °C) and are stable for up to two years. For longer storage, keep all contents at 4 °C. Before use, dissolve the lyophilized RNase A by the addition of 1 mL of Buffer M1. Pipette up and down until the enzyme is dissolved completely. Transfer the RNase A solution into the Buffer M1 bottle and mix thoroughly. Buffer M1 with RNase A should be stored at 4 °C for frequent use and at -20 °C for infrequent use. Buffer M2 may form a precipitate upon storage. If necessary, dissolve the precipitate by warming the solution at 37 °C.
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