Description: Kpn I is a Restriction Endonuclease purified from an Escherichia coli strain that carries the Kpn I gene from Klebsiella pneumonia. Kpn I activity is not affected by CG methylase. NZYTech provides Kpn I with a specific buffer, NZYBuffer C. NZYTech’sKpn I is an enzyme that acts at 37 °C. For double digestions please use NZYBuffer U (not provided, MB110).

 

Concentration: 20000 U/mL.

 

Reagents supplied with the enzyme: 10× NZYBuffer C (200 mM Tris, 500 mM Potassium Acetate, 100 mM Magnesium Acetate, 10 mM DTT, pH 7.9).

 

Storage buffer: 10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 200 µg/mL (w/v) BSA, 50% (v/v) glycerol.