Description: Random hexamer mix includes oligonucleotides representing all possible hexamer sequences. Random hexamers are commonly used for priming single-stranded DNA or RNA for extension by DNA polymerase or Reverse transcriptases (NZY Reverse Transcriptase, MB1240, or NZY M-MuLV Reverse Transcriptase, MB0830). During cDNA synthesis, random hexamer will random priming throughout the length of the RNA to generate a cDNA pool containing various lengths of cDNA. With this method, all RNAs present in a population constitute templates for cDNA synthesis. Random primers are sometimes preferred for cDNA that is used in RT-PCR, especially when the PCR primers target a region near the 5’-end of the RNA.

5’- d (NNNNNN)-3’, where N = G, A, T or C


Storage and shipping conditions: Random hexamer mix can be stored for 12 months at -20 °C. Avoid multiple freeze/thaw cycles. Random hexamer is shipped at 4 ºC.

Concentration: 500 µl at 50 ng/µl.
 

ADDITIONAL INFORMATION

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