GelRed™ is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed™ is far more sensitive than EB without requiring a destaining step. GelRed™ and EB have virtually the same spectra (Figure 3), so you can directly replace EB with GelRed™ without changing your existing imaging system.
GelRed™ can be used to stain dsDNA, ssDNA or RNA in agarose gels via either precast or post gel staining. GelRed™ can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gels via post gel staining. GelRed™ is also compatible with downstream DNA manipulations such as restriction digest, sequencing, and cloning.
A series of safety tests have confirmed that GelRed™ is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, GelRed™ can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal. For detailed test results, you may download the GelRed/GelGreen Safety Report.
GelRed™ 3X in water is ready-to-use for post-electrophoresis gel staining, and is supplied in a 4L cubitainer. Biotium also offers GelRed™ 10,000X solution in water (41003).
FEATURES
Flyer (PDF): GelRed & GelGreen Flyer
MSDS (PDF): MSDS 41001
Protocol (PDF): PI-41001
Safety Report (PDF): GR & GG safety